Measurement
M_VPS4

Part:BBa_K5088510:Design

Designed by: Dascha Khalfine   Group: iGEM24_Marburg   (2024-07-31)


Measurement construct - VPS4 promoter+5'UTR from T. kok-saghyz


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 487
    Illegal XbaI site found at 3706
    Illegal PstI site found at 4133
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 487
    Illegal PstI site found at 4133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 487
    Illegal BglII site found at 3709
    Illegal XhoI site found at 2953
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 487
    Illegal XbaI site found at 3706
    Illegal PstI site found at 4133
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 9
    Illegal EcoRI site found at 487
    Illegal XbaI site found at 3706
    Illegal PstI site found at 4133
    Illegal NgoMIV site found at 3181
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3395


Design Notes

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Source

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References